Western Blot
SPL Smart Protein Layers
Kit for easy normalization of Western blots
The SPL kit enables the simple but very precise detection of how much total protein of a sample is present on the blot membrane at the time of target detection. The ratio of target signal to its corresponding total protein is used for exact normalization. Staining of the membrane or quantitative detection of endogenous proteins (house-keeping proteins) is therefore not necessary. Further SPL features are:
- Highly sensitive protein visualization of gels and blots without staining
- Simple but accurate normalization of Western blots
- further features with additional SPL standards:
- Loading of samples without protein determination
- Monitoring every step of the Western blot analysis
- Template for precise quantification of target signals
- Exact comparisons of data from different experiments
ChemoStar Touch basic system consisting of:
- Scientific grade camera with Schneider-Kreuznach lens
- Robust metal housing with internal focus incident light
- Pull-out sample drawer for sample positioning
- Control unit (PC incl. large 21.5″ touch display)
- Image capture software
- Warranty 12 months
optional for an extra charge
- Fluorescence modules (incl. excitation unit, emission filter and filter wheel)
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
red (Cy5) and NIR (Cy7, Alexa 790)
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
- digital thermal printer
- White light transilluminator
- Blue/green transilluminator
- UV transilluminator
- low-fluorescent sample tray
- Cutting plates for cutting out yellow bands
CCD camera
- 16 bit/ 65,536 Scientific Scientific grade camera
- 6.1 megapixels (2,750 x 2,200 pixels)
- Quantum efficiency (QE) at
525 nm ≈ 75%, 575 nm ≈ 77%, 665 nm ≈ 67%, 750 nm ≈ 60% - dynamic range of 4 decades
- Pixel size 4.54 x 4.54 μm per pixel
- 19,000e- Full well capacity (1 x 1 binning)
- Binning modes: 1 x 1; 2 x 2; 3 x 3; 4 x 4; 5 x 5
- Multi-stage Peltier cooling (ΔT -35 °C)
- Pre-calibration for illumination and image detection
Lens/ Filter
- Highly coated, fast Schneider-Kreuznach lens (f 0.95 / 25 mm)
- Manual focus and automatic exposure (auto exposure)
- High-quality, motorized filter wheel
- Contain filters for the detection of nucleic acids
- Fluorescence modules (incl. excitation unit and emission filter)
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
red (Cy5) and NIR (Cy7, Alexa 790) – optional
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
Control unit
- Powerful mini PC on the rear panel
- Operating system Windows 10 IoT Enterprise LTSC Entry (64 bit)
- 256 GB internal hard disk space
- USB ports for printer, mouse, keyboard, etc.
- Preparation for network connection
- Optional at extra cost: W-Lan interface
- 21.5″ touch display
Other specifications
- Voltage: 100 – 240 V
- Rated frequency: 50/60 Hz
- Rated power: 250 W
- Dimensions: approx. 60 x 50 x 65 cm
UV transilluminator
- Excitation maximum at 312 nm, suitable for ethidium bromide and substitutes such as HDGreen, Gel Red
- Adjustable light intensity
- Illuminated area 21 x 26 cm
- Special UV filter: BP 590 nm
- UV privacy screen set in metal, adjustable
- UV circuit breaker
- Portable (stand alone) system
We recommend:
A blue/green transilluminator can be used instead of a UV transilluminator. The function corresponds to the UV model but is more suitable in terms of occupational safety.
Blue/Green transilluminator
- 505 nm, for ethidium bromide and substitutes such as HDGreen, GelRed
- Adjustable privacy screen set in a metal frame
- Illuminated area 16 x 20 cm
- Circuit breaker
- Portable (stand alone) system
White light transilluminator
- for VIS samples, such as Coomassie or silver-colored gels
- Illuminated area 22 x 16 cm
- Battery-operated (rechargeable with USB)
- Adjustable intensity
- Portable (stand alone) system
for the absorption of 1D gels (RNA/DNA, proteins)
- Personalized registration possible
- Self-explanatory workflow mode
- Exact setting of the region of interest (ROI)
- Auto exposure time for fast measurement with the widest possible dynamic range
- Manual setting of all parameters possible
- Prescan in 4 x 4 binning for quick manual adjustment of the exposure time
- Image export of 16-bit Tiff formats for data processing (raw data) or storage in 8-bit JPEG format (e.g. for lab books)
for the evaluation of 1D gels (RNA/DNA, proteins) – optional
- for Win-PC or MacOS
- Evaluation of 1D gels (DNA / RNA, protein gels)
- 3D gel and blot view
- Clear software interface with image capture, histogram and data table
- Self-explanatory software workflow mode in the function window
- Pre-processing of images (invert, adjust brightness and contrast, colorize and sharpen display image
) - Defined selection possible by defining the ROI
- Automatic and manual lane detection (including angled and curved lanes)
- Automatic and manual band detection (including angled and curved bands)
- Background print
- Precise evaluation with the help of specific tools:
o Rf calibration
o Molecular weights
o Quantification
o Normalization - Clear presentation of e.g. lane volume, band volume, Rf value and molecular weights in the data table
- Definition of various standards (you can also create your own standards)
- Data export to e.g. Excel possible
- Additional applications (line assays and lateral flow) possible at an extra charge
- Extensions (e.g. SPL-Comperator) possible for an additional charge
ChemoStar Plus
The ChemoStar Plus is identical to the ChemoStar Touch, but uses an external PC and monitor. This system is a powerful and faithful companion for Western blots and other chemiluminescence and fluorescence detections. In addition to its robust all-metal construction, special features include
- its very intense and homogeneous fluorescence excitation from 320 nm (UV) to 780 mn (NIR) over an area of 400 cm2
- high sensitivity and dynamic range in detection thanks to the latest CCD/CMOS technologies in combination with powerful optics
- its simple operation
- the modular and flexible design
- its robust design, which allows upgrades and conversions many years later
ChemoStar Plus basic system consisting of:
- Scientific grade camera with Schneider-Kreuznach lens
- Robust metal housing with internal focus incident light
- Pull-out sample drawer for sample positioning
- Control unit (PC incl. large 24″ monitor)
- Image capture software
- Warranty 12 months
optional for an extra charge
- Fluorescence modules (incl. excitation unit, emission filter and filter wheel)
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
red (Cy5) and NIR (Cy7, Alexa 790) – optional
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
- digital thermal printer
- White light transilluminator
- Blue/green transilluminator
- UV transilluminator
- low-fluorescent sample tray
- Cutting plates for cutting out yellow bands
CCD camera
- 16 bit/ 65,536 Scientific grade camera
- 6.1 megapixels (2,750 x 2,200 pixels)
- Quantum efficiency (QE) at
525 nm ≈ 75%, 575 nm ≈ 77%, 665 nm ≈ 67%, 750 nm ≈ 60% - dynamic range of 4 decades
- Pixel size 4.54 x 4.54 μm per pixel
- 19,000e- Full well capacity (1 x 1 binning)
- Binning modes: 1 x 1; 2 x 2; 3 x 3; 4 x 4; 5 x 5
- Multi-stage Peltier cooling (ΔT -35 °C)
- Pre-calibration for illumination and image detection
Lens/ Filter
- Highly coated, fast Schneider-Kreuznach lens (f 0.95 / 25 mm)
- Manual focus and automatic exposure (auto exposure)
- High-quality, motorized filter wheel
- Contain filters for the detection of nucleic acids
- Fluorescence modules (incl. excitation unit and emission filter)
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
red (Cy5) and NIR (Cy7, Alexa 790) – optional
- up to 4 pieces e.g. blue (Cy2, Alexa 488), green (Cy3, TAMRA),
Control unit
- powerful PC
- Operating system Windows 10 IoT Enterprise LTSC Value (64 bit)
- 256 GB internal hard disk storage (SSD)
- USB ports for printer, mouse, keyboard, etc.
- Preparation for network connection
- Optional at extra cost: W-Lan interface
- 24″ monitor
- incl. Mouse and keyboard
Other specifications
- Voltage: 100 – 240 V
- Rated frequency: 50/60 Hz
- Rated power: 250 W
- Dimensions: approx. 60 x 50 x 65 cm
UV transilluminator
- Excitation maximum at 312 nm, suitable for ethidium bromide and substitutes such as HDGreen, Gel Red
- Adjustable light intensity
- Illuminated area 21 x 26 cm
- Special UV filter: BP 590 nm
- UV privacy screen set in metal, adjustable
- UV circuit breaker
- Portable (stand alone) system
We recommend:
A blue/green transilluminator can be used instead of a UV transilluminator. The function corresponds to the UV model but is more suitable in terms of occupational safety.
Blue/Green transilluminator
- 505 nm, for ethidium bromide and substitutes such as HDGreen, GelRed
- Adjustable privacy screen set in a metal frame
- Illuminated area 16 x 20 cm
- Circuit breaker
- Portable (stand alone) system
White light transilluminator
- for VIS samples, such as Coomassie or silver-colored gels
- Illuminated area 22 x 16 cm
- Battery-operated (rechargeable with USB)
- Adjustable intensity
- Portable (stand alone) system
for the absorption of 1D gels (RNA/DNA, proteins)
- Personalized registration possible
- Self-explanatory workflow mode
- Exact setting of the region of interest (ROI)
- Auto exposure time for fast measurement with the widest possible dynamic range
- Manual setting of all parameters possible
- Prescan in 4 x 4 binning for quick manual adjustment of the exposure time
- Image export of 16-bit Tiff formats for data processing (raw data) or storage in 8-bit JPEG format (e.g. for lab books)
for the evaluation of 1D gels (RNA/DNA, proteins) – optional
- for Win-PC or MacOS
- Evaluation of 1D gels (DNA / RNA, protein gels)
- 3D gel and blot view
- Clear software interface with image capture, histogram and data table
- Self-explanatory software workflow mode in the function window
- Pre-processing of images (invert, adjust brightness and contrast, colorize and sharpen display image
) - Defined selection possible by defining the ROI
- Automatic and manual lane detection (including angled and curved lanes)
- Automatic and manual band detection (including angled and curved bands)
- Background print
- Precise evaluation with the help of specific tools:
o Rf calibration
o Molecular weights
o Quantification
o Normalization - Clear presentation of e.g. lane volume, band volume, Rf value and molecular weights in the data table
- Definition of various standards (you can also create your own standards)
- Data export to e.g. Excel possible
- Additional applications (line assays and lateral flow) possible at an extra charge
- Extensions (e.g. SPL-Comperator) possible for an additional charge
Configuration options
The protein sample is labeled before electrophoresis by adding fluorescent smart labels. The now fluorescent proteins can thus be detected in the gel and on the blot (approx. 100x more sensitive than e.g. Coomassie). For the precise normalization of Western blots, the following is used as usual a) the amount of target signal is detected (e.g. via fluorescent secondary antibodies or ECL) and b) the amount of sample (total protein) on the blot at that time is detected.
Depending on your type of target detection and your fluorescence imaging capabilities, we offer different SPL kits to visualize the total protein.
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Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.